Clapton provided 75 guitars and 55 amps to the event, which raised money for The Crossroads Centre in Antigua - a drug rehabilitation centre. The 65-year-old musician co-founded the facility in 1998.
The Bonhams auction was carried out simultaneously in New York, Los Angeles, London and online - and the proceeds more than tripled expectations.
reported that the item which sold for the highest price was a 1948 Gibson hollow-body guitar which sold for $83,000 (£51,300).
A replica of Clapton's famous Fender Stratocaster, known as "Blackie", brought in more than $30,000. The original was sold at auction for a little less than $1million (£600,000) seven years ago.
reported that a Gianni Versace three-piece suit made for Clapton's concert at the Royal Albert Hall in 1990, a black leather tote bag designed by Andrea Valentini and an autographed mobile phone were also included in the musician's contribution to the auction.
called the sale of the 1979 Danny Ferrington three-quarter-sized guitar the biggest surprise of the evening. Its highest estimated value was no more than $500 (£310) and it sold for $42,000 (£26,149).
A hand-written and signed note from Ferrington accompanied the guitar.
Guitars donated and autographed by other musicians, including Jeff Beck, J.J. Cale and Joe Bonamassa, were also sold.
"Bidders from all four corners of the globe battled throughout the 4.5 hour auction and included clients from the U.S., Asia, Australasia, UK, Europe, and the Caribbean," stated a Bonhams
press release. "Notably, a private Russian Eric Clapton fan and collector showed keen interest in the room and Asian participants included those from Japan, Korea and Hong Kong."
Clapton donated instrument for previous fundraising auctions for the centre in 1999 and in 2004.