Singing hymns as they marched, many of them wore t-shirts stating "Christian and Proud" while others adorned themselves with stickers quoting from the Bible, "No fear in love".
Peter Crawford of Young Lesbian and Gay Christians (YGLC) said the Christian turnout at Pride shows how Christian opinion on lesbian, gay, bisexual and transgender (LGBT) people is changing.
"Our presence here today has shown that we represent many, many Christians who fully support LGBT people and their God-given identity as such," Crawford told Ekklesia
After the march, Christians gathered for a communion service at St-Martin-in-the-Fields' Church, led by the Rev Clare Herbert with the sermon given by Rev Giles Goddard.
"I give thanks for Pride, for you, for us and above all for the super-abundant love of Jesus, which enables us to stand tall and walk tall into another year," Goddard said in his sermon.
The Christian presence at Pride was organsied by a coalition of pro-gay Christian groups including Changing Attutide, Courage UK, the Evangelical Fellowship of Lesbian and Gay Christians, and the Lesbian and Gay Chrstian Movement.