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article imageFemale Plant 'Communicates' Rejection or Acceptance of Male

By Bob Ewing     Oct 25, 2008 in Science
University of Missouri researchers have identified pollen proteins that may contribute to the signaling processes that determine if a plant accepts or rejects individual pollen grains for reproduction.
A plant's main purpose is to reproduce and selecting a partner,even for a plant, can be a challenging task.
University of Missouri researchers have identified pollen proteins that may contribute to the signaling processes that determine if a plant accepts or rejects individual pollen grains for reproduction.
Plants rely on external factors such as wind and animals to bring them potential mates in the form of pollen grains. When pollen grains arrive, an introduction occurs through a “conversation” between the pollen (the male part of the flower) and the pistil (the female part of the flower).
The plants use molecules rather than words and this allows the pollen to identify itself to the pistil. If we eavesdrop on this molecular conversation it may provide ways to control the spread of transgenes from genetically-modified crops to wild relatives, offer better ways to control fertilization between cross species, and lead to a more efficient way of growing fruit trees.
“Unlike an animal’s visual cues about mate selection, a plant’s mate recognition takes place on a molecular level,” said Bruce McClure, associate director of the Christopher S. Bond Life Sciences Center and researcher in the MU Interdisciplinary Plant Group and Division of Biochemistry
. “The pollen must, in some way, announce to the pistil its identity, and the pistil must interpret this identity. To do this, proteins from the pollen and proteins from the pistil interact; this determines the acceptance or rejection of individual pollen grains.”
Researchers used two specific pistil proteins, NaTTS and 120K, as “bait” to see what pollen proteins would bind to them. These two pistil proteins were used because they directly influence the growth of pollen down the pistil to the ovary where fertilization takes place.
Three proteins, S-RNase-binding protein (SBP1), the protein NaPCCP and an enzyme, bound to the pistil proteins. This action suggests that these proteins likely contribute to the signaling processes that affect the success of pollen growth.
“Our experiment was like putting one side of a Velcro strip on two pistil proteins and then screening a collection of pollen proteins to see which of the pollen proteins have the complementary Velcro strip for binding,” McClure said.
“If it sticks, it’s a good indication that the pollen proteins work with the pistil proteins to determine the success of reproduction.”
McClure has previously shown S-RNase, a protein on the pistil side, caused rejection of pollen from close relatives by acting as a cytotoxin, or a toxic substance, in the pollen tube.
The MU team used Nicotiana alata, a relative of tobacco commonly grown in home gardens as “flowering tobacco.” The study, “Pollen Proteins Bind to the C-Terminal Domain of Nicotiana Alata Pistil Arabinogalactan Proteins,” was published in the Journal of Biological Chemistry and was co-authored by McClure; Kirby N. Swatek, biochemistry graduate student; and Christopher B. Lee, post-doctoral researcher at the Bond Life Sciences Center.
Faculty from six of MU’s colleges and schools perform interdisciplinary research in the Christopher S. Bond Life Sciences Center with a vision to become a recognized world-wide center of scientific excellence and leadership in life sciences research, innovation and education.
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