Gene Reduces Harmful Carcinogens in Tobacco

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A recent discovery by scientists from North Carolina State University may lead the way to tobacco products, especially smokeless products, which have reduced amounts of cancer-causing agents.

The press release says that in large-scale field trials researchers have shown that silencing a specific gene in burley tobacco plants significantly reduces harmful carcinogens in cured tobacco leaves.

NC State's Dr. Ralph Dewey, professor of crop science, and Dr. Ramsey Lewis, assistant professor of crop science, teamed with colleagues from the University of Kentucky to knock out a gene known to turn nicotine into nornicotine.

Nornicotine is a precursor to the carcinogen N-nitrosonornicotine (NNN). Varying percentages of nicotine are turned into nornicotine while the plant ages; nornicotine converts to NNN as the tobacco is cured, processed and stored.

Field tests were conducted in Kentucky, Virginia and North Carolina and compared cured burley tobacco plants with the troublesome gene silenced and "control" plant lines with normal levels of gene expression.

The researchers discovered a six-fold decrease in carcinogenic NNN in the genetically modified tobacco plants, as well as a 50 percent overall reduction in the class of harmful compounds called TSNAs, or tobacco-specific nitrosamines.

TSNAs are reported to be among the most important tobacco-related compounds implicated in various cancers in laboratory experiments.

The research results were published online in Plant Biotechnology Journal.

The researchers emphasizes that the best way for people to avoid the risks associated with tobacco use is to avoid using tobacco products. But their findings show that targeted gene silencing can work as well in the field as it does on the lab bench.

"Creating a tobacco plant with fewer or no harmful compounds may also help with tobacco plants that are being used to create pharmaceuticals or other high-value products," Dewey said.

The researchers, in order to get initial lines of plants with the troublesome gene silenced, used a technique called RNA interference in which genetic engineering was used to introduce a gene that inhibits the demethylase gene function into the tobacco plant.

The researchers have since developed tobacco lines with the same effect without using genetic engineering. First, they randomly inserted chemical changes, or mutations, into the tobacco genome of burley tobacco plants.

Then, they searched for plants in which the nicotine demethylase gene was permanently impaired. The researchers are currently working to transfer this mutation to widely used tobacco varieties.


Nothing else in the plant changed, growth or resistance to insects or disease, for example, after they knocked out this specific gene.

While Lewis believes that varieties of burley tobacco with a silenced demethylase gene will exist within the next few years, the researchers say burley tobacco has a number of other targets for their gene silencing method.

The research is sponsored by Philip Morris USA.